Microfluidically-Assisted Isolation and Characterization of Achromobacter spanius from Soils for Microbial Degradation of Synthetic Polymers and Organic Solvents

A micro segmented-flow approach was utilized for the isolation soil bacteria that can degrade synthetic polymers as polyethylene glycols (PEG) and polyacrylamide (PAM). We had been able to obtain many strains; among them, five Achromobacter spanius strains from samples of specific sampling sites were connected with ancient human impacts. In addition characterization community responses isolating single strains, this microfluidic allowed investigation susceptibility against three polymers, including PEG, PAM, Polyvinylpyrrolidone (PVP) two organic solvents known 1,4-dioxane diglyme. The small stepwise variation effector concentrations in 500 nL droplets provides a detailed reflection concentration-dependent response bacterial growth endogenous autofluorescence activity. As result, all use PEG600 carbon source. Furthermore, showed similar dose-response characteristics However, significantly different PAM- PVP-tolerances found these strains. Samples surface prehistorical rampart areas supplied strain capable degradation PVP, PAM. This study demonstrates on one hand, potential microsegment flow miniaturized screening studies its ability detect novel other isolated may be useful providing optimal conditions bioremediation biodegradation processes.